1V9M
Crystal structure of the C subunit of V-type ATPase from Thermus thermophilus
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 90 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-10-02 |
| Detector | RIGAKU RAXIS V |
| Wavelength(s) | 0.9793, 0.9795, 0.9781 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.790, 83.806, 48.567 |
| Unit cell angles | 90.00, 113.86, 90.00 |
Refinement procedure
| Resolution | 19.620 - 1.850 |
| Rwork | 0.202 |
| R-free | 0.22800 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.000 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.061 | 0.338 |
| Number of reflections | 25341 | |
| <I/σ(I)> | 20 | 3.7 |
| Completeness [%] | 97.1 | 91.5 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | PEG 300, PEG 8000, glycerol, Tris-HCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
