1V9F

Crystal structure of catalytic domain of pseudouridine synthase RluD from Escherichia coli

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2003-10-29
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9000, 0.9788, 0.9795, 0.9814
Spacegroup name	P 65 2 2
Unit cell lengths	74.506, 74.506, 265.009
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.490 - 1.700
R-factor	0.20094
Rwork	0.200
R-free	0.22420
Structure solution method	MAD
RMSD bond length	0.013
RMSD bond angle	1.452
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.1.19)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.058	0.241
Number of reflections	47799
<I/σ(I)>	18.1
Completeness [%]	98.7	91
Redundancy	15.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	Ethylene glycol, PEG3000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K