1V9E

Crystal Structure Analysis of Bovine Carbonic Anhydrase II

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	100
Detector technology	CCD
Collection date	2001-03-08
Detector	ADSC QUANTUM 4
Wavelength(s)	1.00
Spacegroup name	P 61
Unit cell lengths	66.700, 66.700, 240.000
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	17.870 - 1.950
Rwork	0.270
R-free	0.29800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005 *
RMSD bond angle	1.420 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	EPMR
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.060
High resolution limit [Å]	1.950	1.950
Rmerge	0.076 *	0.284
Total number of observations	213573 *
Number of reflections	35487 *
<I/σ(I)>	6.4	2.3
Completeness [%]	90.6	90.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *	7.5	277	50mM Tris-HCl, 2.4M Ammonium sulfate, pH 7.5, SMALL TUBES, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	17 (mg/ml)
2	1	1	Tris	50 (mM)	pH7.5
3	1	1	ammonium sulfate	2.4 (M)