1V96
Crystal structure of hypothetical protein of unknown function from pyrococcus horikoshii OT3
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-10-23 |
| Detector | RIGAKU RAXIS V |
| Wavelength(s) | 0.9792, 0.9795, 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.909, 95.463, 102.656 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.750 |
| R-factor | 0.209 |
| Rwork | 0.209 |
| R-free | 0.22800 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 2.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.039 | 0.199 |
| Number of reflections | 45126 | |
| Completeness [%] | 99.2 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 295 | 6.4% PEG 4000, 0.1M CITRATE, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
