1V8Z

X-ray crystal structure of the Tryptophan Synthase b2 Subunit from Hyperthermophile, Pyrococcus furiosus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Detector	Bruker DIP-6040
Wavelength(s)	0.9
Spacegroup name	P 21 21 21
Unit cell lengths	84.835, 110.530, 159.999
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	67.300 - 2.210
Rwork	0.208
R-free	0.26300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bks
RMSD bond length	0.006
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall
Low resolution limit [Å]	67.300
High resolution limit [Å]	2.210
Rmerge	0.053
Number of reflections	75098
Completeness [%]	98.7
Redundancy	9.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	283	PEG20000, MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 283K