1V8R

Crystal structure analysis of the ADP-ribose pyrophosphatase complexed with ADP-ribose and Zn

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44B2
Synchrotron site	SPring-8
Beamline	BL44B2
Detector technology	CCD
Detector	MARRESEARCH
Wavelength(s)	1.282
Spacegroup name	P 32 2 1
Unit cell lengths	49.710, 49.710, 118.140
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.020 - 1.800
R-factor	0.206
Rwork	0.206
R-free	0.23500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.300
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.860
High resolution limit [Å]	1.800	1.800
Number of reflections	16237
Completeness [%]	99.2	99.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	VAPOR DIFFUSION, HANGING DROP, temperature 293K