1V8Q

Crystal structure of ribosomal protein L27 from Thermus thermophilus HB8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44B2
Synchrotron site	SPring-8
Beamline	BL44B2
Temperature [K]	100
Detector technology	CCD
Collection date	2002-02-25
Detector	MACSCIENCE
Wavelength(s)	0.90
Spacegroup name	P 43
Unit cell lengths	48.543, 48.543, 139.120
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.830 - 2.800
R-factor	0.197
Rwork	0.197
R-free	0.23600
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.000
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.830	2.900
High resolution limit [Å]	2.800	2.800
Number of reflections	7947
<I/σ(I)>	27.8	3.6
Completeness [%]	99.7	97.3
Redundancy	7.5	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	303	PEG 4000, Ammonium Acetate, tri-Sodium Citrate dihydrate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 303K
1	VAPOR DIFFUSION, HANGING DROP	6	303	PEG 4000, Ammonium Acetate, tri-Sodium Citrate dihydrate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 303K