1V8J

The Crystal Structure of the Minimal Functional Domain of the Microtubule Destabilizer KIF2C Complexed with Mg-ADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	95
Detector technology	CCD
Collection date	2003-10-15
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9800
Spacegroup name	C 2 2 2
Unit cell lengths	61.209, 192.453, 73.554
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 * - 3.240
R-factor	0.248
Rwork	0.248
R-free	0.29200 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012 *
RMSD bond angle	2.100 *
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000 *	3.440
High resolution limit [Å]	3.240	3.240
Rmerge	0.110 *
Number of reflections	6296
Completeness [%]	92.2 *	76.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	24 *	potassium sodium tartrate tetrahydrate, MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 297.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	potassium sodium tartrate tetrahydrate	1.0 (M)
3	1	reservoir	MES	0.1 (M)	pH6.0