1V8I

Crystal Structure Analysis of the ADP-ribose pyrophosphatase

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44B2
Synchrotron site	SPring-8
Beamline	BL44B2
Temperature [K]	103
Detector technology	CCD
Detector	MARRESEARCH
Wavelength(s)	0.9804, 0.9808, 0.9763, 0.9822
Spacegroup name	P 32 2 1
Unit cell lengths	49.592, 49.592, 117.915
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.000 - 1.760
R-factor	0.213
Rwork	0.213
R-free	0.23600
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.820
High resolution limit [Å]	1.760	1.760
Number of reflections	17390

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	PEG 4000, ammonium sulfate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K