1V8H
Crystal structure of TT0351 protein from Thermus thermophilus HB8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-02-20 |
| Detector | RIGAKU RAXIS V |
| Wavelength(s) | 0.80 |
| Spacegroup name | P 65 |
| Unit cell lengths | 84.759, 84.759, 59.882 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 1.200 |
| R-factor | 0.2116 |
| Rwork | 0.211 |
| R-free | 0.21270 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.240 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.039 | 0.419 |
| Number of reflections | 76116 | |
| <I/σ(I)> | 15.4 | |
| Completeness [%] | 99.6 | 98.3 |
| Redundancy | 4.8 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 6.1 | 291 | sodium citrate, pH 6.1, microbatch, temperature 291K |
