1V8G
Crystal structure of anthranilate phosphoribosyltransferase (TrpD) from Thermus thermophilus HB8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-10-24 |
| Detector | RIGAKU JUPITER |
| Wavelength(s) | 1.00 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 143.318, 72.450, 73.539 |
| Unit cell angles | 90.00, 110.36, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.100 |
| R-factor | 0.212 |
| Rwork | 0.212 |
| R-free | 0.25500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kgz |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.072 | 0.255 |
| Number of reflections | 41300 | |
| <I/σ(I)> | 15.2 | 3.17 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 3.65 | 3.56 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | microbach | 5.8 | 291 | 27.5 w/v PEG4000, 0.1M citrate, pH 5.8, microbach, temperature 291K |
