1V8E
Crystal Structure of Glycerophosphoryl Diester Phosphodiesterase from Thermus thermophilus HB8
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL45PX |
Synchrotron site | SPring-8 |
Beamline | BL45PX |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2002-04-21 |
Detector | RIGAKU RAXIS V |
Wavelength(s) | 1.009 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 74.221, 74.221, 73.518 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.390 - 1.500 |
R-factor | 0.2075 |
Rwork | 0.205 |
R-free | 0.23490 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.550 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.083 | 0.543 |
Number of reflections | 36456 | |
<I/σ(I)> | 8 | 2 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 6.27 | 5.65 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 8.1 | 291 | ammonium sulfate, dioxane, pH 8.1, Microbatch, temperature 291K |
1 | MICROBATCH | 8.1 | 291 | ammonium sulfate, dioxane, pH 8.1, Microbatch, temperature 291K |
1 | MICROBATCH | 8.1 | 291 | ammonium sulfate, dioxane, pH 8.1, Microbatch, temperature 291K |