1V8E
Crystal Structure of Glycerophosphoryl Diester Phosphodiesterase from Thermus thermophilus HB8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45PX |
| Synchrotron site | SPring-8 |
| Beamline | BL45PX |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-04-21 |
| Detector | RIGAKU RAXIS V |
| Wavelength(s) | 1.009 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 74.221, 74.221, 73.518 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.390 - 1.500 |
| R-factor | 0.2075 |
| Rwork | 0.205 |
| R-free | 0.23490 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.083 | 0.543 |
| Number of reflections | 36456 | |
| <I/σ(I)> | 8 | 2 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 6.27 | 5.65 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 8.1 | 291 | ammonium sulfate, dioxane, pH 8.1, Microbatch, temperature 291K |
| 1 | MICROBATCH | 8.1 | 291 | ammonium sulfate, dioxane, pH 8.1, Microbatch, temperature 291K |
| 1 | MICROBATCH | 8.1 | 291 | ammonium sulfate, dioxane, pH 8.1, Microbatch, temperature 291K |
