1V7Y
Crystal structure of tryptophan synthase alpha-subunit from Escherichia coli at room temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-07-01 |
| Detector | MAC Science DIP-2000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 158.420, 45.100, 72.060 |
| Unit cell angles | 90.00, 96.90, 90.00 |
Refinement procedure
| Resolution | 19.560 - 2.500 |
| R-factor | 0.218 |
| Rwork | 0.218 |
| R-free | 0.27100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.560 | 2.660 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 15954 | |
| Completeness [%] | 89.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 288 | Ammonium sulfate, Sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
