1V7X
Crystal structure of Vibrio proteolyticus chitobiose phosphorylase in complex with GlcNAc and sulfate
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-10-17 |
| Detector | ACSC QUANTUM 210 |
| Wavelength(s) | 1.0000, 0.97120, 0.97848, 0.97960 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 140.871, 70.355, 79.939 |
| Unit cell angles | 90.00, 98.36, 90.00 |
Refinement procedure
| Resolution | 47.690 - 2.000 |
| R-factor | 0.163 |
| Rwork | 0.163 |
| R-free | 0.19500 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.087 | 0.271 |
| Number of reflections | 52164 | |
| <I/σ(I)> | 9.5 | 3.31 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 1.9 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | PEG400, HEPES, Calcium chloride, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
