1V7V
Crystal structure of Vibrio proteolyticus chitobiose phosphorylase
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-06-23 |
Detector | ACSC QUANTUM 210 |
Wavelength(s) | 1.0000, 0.97120, 0.97848, 0.97960 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 140.946, 70.580, 80.045 |
Unit cell angles | 90.00, 98.40, 90.00 |
Refinement procedure
Resolution | 47.780 - 1.800 |
R-factor | 0.162 |
Rwork | 0.162 |
R-free | 0.18600 |
Structure solution method | MAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.049 | 0.228 |
Number of reflections | 70781 | |
<I/σ(I)> | 15.3 | 5.33 |
Completeness [%] | 98.5 | 97.6 |
Redundancy | 2 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | PEG400, HEPES, Calcium chloride, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |