1V7U

Crystal structure of Undecaprenyl Pyrophosphate Synthase with farnesyl pyrophosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	123
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5
Spacegroup name	P 21 21 21
Unit cell lengths	63.030, 69.200, 108.260
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.350
R-factor	0.254
Rwork	0.173
R-free	0.25400
Structure solution method	AB INITIO PHASING
RMSD bond length	0.021
RMSD bond angle	1.870
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000	2.430
High resolution limit [Å]	2.350	2.350
Rmerge	0.129	0.363 *
Total number of observations	95659 *
Number of reflections	19508	1882 *
<I/σ(I)>	11	11
Completeness [%]	97.1	95.1 *
Redundancy	4.65	4.65

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5 *	25 *	25% ethylene glycol, 0.05% triton, 0.5mM MgCl2, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ethylene glycol	25 (%)	pH7.5
2	1	drop	protein	10 (mg/ml)
3	1	drop	peptide	1 (mM)
4	1	drop	Triton X-100	0.05 (%)
5	1	drop		0.5 (mM)