1V7T
Triclinic lysozyme with low solvent content obtained by phase transition
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | MACSCIENCE |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2002-04-11 |
| Detector | BRUKER SMART 6000 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 25.930, 39.970, 42.390 |
| Unit cell angles | 88.00, 95.44, 90.58 |
Refinement procedure
| Resolution | 39.940 - 1.130 |
| R-factor | 0.119 |
| Rwork | 0.114 |
| R-free | 0.16200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 0.028 |
| Data reduction software | SaintPlus |
| Data scaling software | SAINTPLUS |
| Phasing software | X-PLOR |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.940 | 1.170 |
| High resolution limit [Å] | 1.130 | 1.130 |
| Rmerge | 0.034 | 0.138 |
| Number of reflections | 52044 | |
| <I/σ(I)> | 3.65 | |
| Completeness [%] | 80.6 | 46.4 |
| Redundancy | 3.18 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | 313 | sodium nitrate, sodium acetate, pH 4.5, phase transition, temperature 313K |
