1V7S
Triclinic hen lysozyme crystallized at 313K from a D2O solution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | MACSCIENCE |
| Temperature [K] | 290 |
| Detector technology | CCD |
| Collection date | 2001-03-06 |
| Detector | BRUKER SMART 6000 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 27.233, 31.967, 34.272 |
| Unit cell angles | 88.44, 108.62, 111.71 |
Refinement procedure
| Resolution | 17.090 - 1.140 |
| R-factor | 0.09 |
| Rwork | 0.087 |
| R-free | 0.12000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 0.031 |
| Data reduction software | SaintPlus |
| Data scaling software | SAINTPLUS |
| Phasing software | X-PLOR |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.090 | 1.160 |
| High resolution limit [Å] | 1.140 | 1.140 |
| Rmerge | 0.038 | 0.080 |
| Number of reflections | 36551 | |
| Completeness [%] | 95.6 | 91.6 |
| Redundancy | 3.49 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION, RECRYSTALLIZATION | 4.5 | 313 | sodium nitrate, sodium acetate, pH 4.5, EVAPORATION, RECRYSTALLIZATION, temperature 313K |
