1V7M
Human Thrombopoietin Functional Domain Complexed To Neutralizing Antibody TN1 Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-12-07 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 132.099, 46.771, 185.151 |
| Unit cell angles | 90.00, 90.48, 90.00 |
Refinement procedure
| Resolution | 15.000 * - 2.500* |
| R-factor | 0.23185 |
| Rwork | 0.228 |
| R-free | 0.31500 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iai |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.000 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.074 * | 0.318 * |
| Total number of observations | 120117 * | |
| Number of reflections | 36766 | |
| <I/σ(I)> | 8.1 | 1.1 |
| Completeness [%] | 92.0 | 71 |
| Redundancy | 2.6 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 295 * | Kuroki, R., (2002) Acta Crystallogr.,Sect.D, 58, 856. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 18-20 (mg/ml) | |
| 2 | 1 | drop | imidazole | 20 (mM) | pH7.0 |
| 3 | 1 | reservoir | PEG3350 | 19-21 (%(w/v)) | |
| 4 | 1 | reservoir | potassium phosphate | 100 (mM) | pH6.0 |
