1V7L
Structure of 3-isopropylmalate isomerase small subunit from Pyrococcus horikoshii
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-12-19 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.979131, 0.979359, 0.97001 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 95.208, 95.208, 152.258 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 1.980 |
| Rwork | 0.206 |
| R-free | 0.24700 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.640 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.090 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmerge | 0.084 | 0.389 |
| Number of reflections | 29033 | |
| <I/σ(I)> | 7.1 | 2 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 15.1 | 15.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 293 | 0.1M HEPES, 1.4M AMMONIUM SULFATE, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
