1V76
Crystal Structure of Archaeal Ribonuclease P Protein Ph1771p from Pyrococcus horikoshii OT3
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-10-30 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97972, 0.90 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 24.133, 56.271, 71.756 |
| Unit cell angles | 90.00, 96.80, 90.00 |
Refinement procedure
| Resolution | 30.100 - 2.000 |
| R-factor | 0.209 |
| Rwork | 0.209 |
| R-free | 0.25700 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.096 | 0.240 |
| Number of reflections | 25204 | |
| <I/σ(I)> | 14.6 | 8.8 |
| Completeness [%] | 99.7 | 99.1 |
| Redundancy | 3.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 1.0M ammonium sulfate, 100mM Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
