1V74
Structure of the E. coli colicin D bound to its immunity protein ImmD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-09-03 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.99 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 62.057, 62.057, 147.934 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| Rwork | 0.194 |
| R-free | 0.23400 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.100 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 2.020 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Number of reflections | 20818 | |
| <I/σ(I)> | 18.9 | 1.6 |
| Completeness [%] | 98.3 | 86.7 |
| Redundancy | 13 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 30% PEG 400, 0.2M MgSO4, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
