1V74
Structure of the E. coli colicin D bound to its immunity protein ImmD
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-09-03 |
Detector | MARRESEARCH |
Wavelength(s) | 0.99 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 62.057, 62.057, 147.934 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.000 |
Rwork | 0.194 |
R-free | 0.23400 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.100 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 99.000 | 2.020 |
High resolution limit [Å] | 1.980 | 1.980 |
Number of reflections | 20818 | |
<I/σ(I)> | 18.9 | 1.6 |
Completeness [%] | 98.3 | 86.7 |
Redundancy | 13 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 30% PEG 400, 0.2M MgSO4, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 291K |