1V73
Crystal Structure of Cold-Active Protein-Tyrosine Phosphatase of a Psychrophile Shewanella SP.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | SIEMENS |
| Temperature [K] | 103 |
| Detector technology | AREA DETECTOR |
| Collection date | 2001-12-28 |
| Detector | SIEMENS HI-STAR |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.503, 76.771, 81.024 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.930 - 1.820 |
| R-factor | 0.179 |
| Rwork | 0.177 |
| R-free | 0.22600 |
| Structure solution method | MIR |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | SADIE |
| Data scaling software | SAINT |
| Phasing software | PHASES |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 14.930 | 1.930 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Number of reflections | 29747 | |
| Completeness [%] | 91.4 | 76.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 30% PEG4000, 0.1M Tris-HCl, 0.2M Ammonium acetate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
