1V6V

Crystal Structure Of Xylanase From Streptomyces Olivaceoviridis E-86 Complexed With 3(2)-alpha-L-arabinofuranosyl-xylotriose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	100
Detector technology	CCD
Collection date	2001-10-01
Detector	ADSC QUANTUM 4
Wavelength(s)	0.978
Spacegroup name	P 21 21 21
Unit cell lengths	78.560, 93.990, 139.130
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.970 - 2.100
R-factor	0.174
Rwork	0.174
R-free	0.20400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1isx
RMSD bond length	0.005
RMSD bond angle	1.300
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.015	2.000
High resolution limit [Å]	1.900	1.900
Rmerge	0.067	0.272
Number of reflections	80990
<I/σ(I)>	9.2	3
Completeness [%]	99.3	100
Redundancy	4	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.7	293	AMMONIUM SULFATE, pH 5.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K