1V6T
Crystal Structure of Lactam Utilization Protein from Pyrococcus horikoshii Ot3
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2003-10-18 |
Detector | RIGAKU RAXIS V |
Wavelength(s) | 1.00, 0.97914, 0.97964 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 65.366, 74.288, 105.559 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.780 - 1.700 |
R-factor | 0.202 |
Rwork | 0.201 |
R-free | 0.22700 |
Structure solution method | MAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.043 | 0.259 |
Number of reflections | 28634 | |
<I/σ(I)> | 20.1 | 6.35 |
Completeness [%] | 100.0 | 100 |
Redundancy | 5.9 | 5.14 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 4.2 | 295 | sodium chloride, acetate, pH 4.2, microbatch, temperature 295K |