1V6O
Peanut lectin complexed with 10mer peptide (PVRIWSSATG)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 128.127, 125.800, 84.740 |
| Unit cell angles | 90.00, 116.19, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.000 |
| R-factor | 0.174 |
| Rwork | 0.174 |
| R-free | 0.24100 * |
| Structure solution method | Isomorphous replacement |
| Starting model (for MR) | 1cr7 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 26.500 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.100 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.167 | 0.357 |
| Total number of observations | 110293 * | |
| Number of reflections | 43166 | 1185 * |
| Completeness [%] | 95.5 | 94 |
| Redundancy | 2.6 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.6 | 293 | 30% PEG 8000, 0.2M Ammonium sulfate and 0.1M cacodylate pH6.5, pH 4.6, hanging drop method, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4.2 (mg/ml) | |
| 2 | 1 | drop | sodium acetate | 0.05 (M) | |
| 3 | 1 | drop | 0.2 (M) | ||
| 4 | 1 | drop | sodium azide | 0.05 (%) | |
| 5 | 1 | drop | lactose | 2.1 (mM) | |
| 6 | 1 | reservoir | PEG8000 | 12 (%) |
