1V6J

peanut lectin-lactose complex crystallized in orthorhombic form at acidic pH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	293
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 21 21 2
Unit cell lengths	128.733, 125.531, 76.050
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.900
Rwork	0.161
R-free	0.22200 *
Structure solution method	Isomorphous replacement
RMSD bond length	0.011
RMSD bond angle	27.000 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.920
High resolution limit [Å]	2.900	2.900
Rmerge	0.151	0.528 *
Total number of observations	191532 *
Number of reflections	26610	1304 *
Completeness [%]	97.6	96.5
Redundancy	7.2	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.6	293	PEG 8000, 0.05M sodium acetate buffer, 0.2M NaCl, 0.05% Sodium azide, pH 4.6, hanging drop method, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	4.2 (mg/ml)
2	1	drop	sodium acetate	0.05 (M)
3	1	drop		0.2 (M)
4	1	drop	sodium azide	0.05 (%)
5	1	drop	lactose	2.1 (mM)
6	1	reservoir	PEG8000	12 (%)