1V67

Structure of ferripyochelin binding protein from pyrococcus horikoshii OT3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2003-11-16
Detector	RIGAKU RAXIS
Wavelength(s)	1.5418
Spacegroup name	F 41 3 2
Unit cell lengths	158.511, 158.511, 158.511
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.810 - 2.300
R-factor	0.189
Rwork	0.189
R-free	0.23800
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1v3w
RMSD bond length	0.009
RMSD bond angle	1.500
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.071	0.240
Number of reflections	7997
Completeness [%]	99.5	98.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	295	26% PEG4000, 0.2M CALCIUM CHLORIDE, HEPES-NaOH, 15mM SODIUM BICARBONATE(NaHCO3), pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K