1V5Y
Binding of coumarins to NAD(P)H:FMN oxidoreductase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6A |
Synchrotron site | Photon Factory |
Beamline | BL-6A |
Wavelength(s) | 1 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 101.600, 63.300, 74.400 |
Unit cell angles | 90.00, 100.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.900 |
R-factor | 0.185 |
Rwork | 0.185 |
R-free | 0.22600 |
Structure solution method | FOURIER SYNTHESIS |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | DM |
Refinement software | X-PLOR |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | PEG 4000, sodium acetate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | PEG 4000, sodium acetate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |