1V5X
Crystal structure of Phosphoribosyl anthranilate isomerase from Thermus Thermophilus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2003-10-03 |
Detector | RIGAKU RAXIS V |
Wavelength(s) | 1.00 |
Spacegroup name | P 41 3 2 |
Unit cell lengths | 144.205, 144.205, 144.205 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.000 - 2.000 |
R-factor | 0.264 |
Rwork | 0.262 |
R-free | 0.29600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1nsj |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.080 | 0.763 |
Total number of observations | 307402 * | |
Number of reflections | 35156 | |
<I/σ(I)> | 9.92 | 3 |
Completeness [%] | 99.8 | 99.4 |
Redundancy | 8.7 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Batch method * | 8 * | 22 * | Isopropanol, Sodium Acetate, pH 5.1, microbatch, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 29.63 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 20 (mM) | pH8.0 |
3 | 1 | drop | 0.2 (M) | ||
4 | 1 | reservoir | iso-propanol | 27.5 (%) | |
5 | 1 | reservoir | sodium acetate | 0.1 (M) | pH5.1 |