1V5W
Crystal structure of the human Dmc1 protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44B2 |
Synchrotron site | SPring-8 |
Beamline | BL44B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-06-12 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9821 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 124.091, 124.091, 218.830 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 3.200 |
R-factor | 0.2955 |
Rwork | 0.294 |
R-free | 0.34590 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1n0w |
RMSD bond length | 0.009 |
RMSD bond angle | 1.406 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.310 |
High resolution limit [Å] | 3.200 | 3.200 |
Number of reflections | 14399 | |
Completeness [%] | 99.2 | 96.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 293 | PEG2000MME, Magnesium chloride, Sodium citrate, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |