1V5V

Crystal Structure of a Component of Glycine Cleavage System: T-protein from Pyrococcus horikoshii OT3 at 1.5 A Resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2003-10-17
Detector	RIGAKU RAXIS
Wavelength(s)	2.0, 0.91971, 0.92001, 0.92540
Spacegroup name	P 21 21 21
Unit cell lengths	79.496, 96.099, 118.605
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.500 - 1.500
R-factor	0.197
Rwork	0.197
R-free	0.20900
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.550
High resolution limit [Å]	1.500	1.500
Rmerge	0.049
Number of reflections	141020
Completeness [%]	97.3	96.8
Redundancy	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	5.9	295	PEG, MES, pH 5.9, MICROBATCH, temperature 295K