1V59

Crystal structure of yeast lipoamide dehydrogenase complexed with NAD+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2001-11-11
Detector	OXFORD PX210
Wavelength(s)	0.9
Spacegroup name	P 21 21 21
Unit cell lengths	66.600, 96.400, 160.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.050 - 2.200
Rwork	0.203
R-free	0.24600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.300
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.050	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.095	0.318
Number of reflections	53020
<I/σ(I)>	7.6	2.3
Completeness [%]	99.8	100
Redundancy	7.16	7.34

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	PEG 4000, MPD, HEPES, potassium phosphate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K