1V59
Crystal structure of yeast lipoamide dehydrogenase complexed with NAD+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-11-11 |
Detector | OXFORD PX210 |
Wavelength(s) | 0.9 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 66.600, 96.400, 160.100 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.050 - 2.200 |
Rwork | 0.203 |
R-free | 0.24600 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.050 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.095 | 0.318 |
Number of reflections | 53020 | |
<I/σ(I)> | 7.6 | 2.3 |
Completeness [%] | 99.8 | 100 |
Redundancy | 7.16 | 7.34 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | PEG 4000, MPD, HEPES, potassium phosphate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |