1V58

Crystal Structure Of the Reduced Protein Disulfide Bond Isomerase DsbG

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	2002-08-12
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9, 0.9783, 0.9785, 0.9556
Spacegroup name	C 1 2 1
Unit cell lengths	116.541, 57.186, 85.508
Unit cell angles	90.00, 94.99, 90.00

Refinement procedure

Resolution	26.550 - 1.700
Rwork	0.186
R-free	0.20700
Structure solution method	MAD
RMSD bond angle	0.005
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.550	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.061	0.269
Number of reflections	57306
<I/σ(I)>	30.6	3.9
Completeness [%]	92.7	53.5
Redundancy	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	3.75	293	20% PEG 4000, 0.1M sodium citrate, 0.2M ammonium sulfate, pH 3.75, VAPOR DIFFUSION, HANGING DROP, temperature 293K