1V57

Crystal Structure of the Disulfide Bond Isomerase DsbG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2001-12-06
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	116.518, 57.047, 85.417
Unit cell angles	90.00, 95.01, 90.00

Refinement procedure

Resolution	32.170 - 2.000
Rwork	0.200
R-free	0.23200
Structure solution method	DIFFERENCE FOURIER
Starting model (for MR)	Structure of Reduced DsbG
RMSD bond angle	0.005
Data reduction software	CRYSTAL (CLEAR 1.3)
Data scaling software	CrystalClear (V. 1.3 (MSC/RIGAKU))
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.170	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.081	0.313
Number of reflections	36303
<I/σ(I)>	6.4	2.1
Completeness [%]	95.5	88
Redundancy	3.2	2.87

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	3.75	293	20% PEG 4000, 0.1M sodium citrate, 0.2M ammonium sulfate, pH 3.75, VAPOR DIFFUSION, HANGING DROP, temperature 293K