1V4W
Crystal structure of bluefin tuna hemoglobin deoxy form at pH7.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Temperature [K] | 293 |
| Detector technology | DIFFRACTOMETER |
| Detector | WEISSENBERG |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.766, 58.637, 174.414 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 13.840 - 1.700 |
| R-factor | 0.1758 |
| Rwork | 0.174 |
| R-free | 0.20230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.578 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.22) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.055 | 0.241 |
| Number of reflections | 57398 | |
| <I/σ(I)> | 11.3 | |
| Completeness [%] | 80.5 | 54.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH METHOD | 7.5 | 293 | PEG 2000, sodium phosphate, glycerol, pH 7.5, BATCH METHOD, temperature 293K |
