1V4T
Crystal structure of human glucokinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL32B2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-12-09 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 103.180, 103.180, 281.020 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.430 - 3.400 |
| R-factor | 0.244 |
| Rwork | 0.237 |
| R-free | 0.30700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1v4s |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.200 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | CNX (2002) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 87.710 | 3.580 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Number of reflections | 12925 | |
| <I/σ(I)> | 6.84 | 3.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.1 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.7 | 293 | Ammonium sulfate, Bicine, Sodium chloride, pH 8.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
