1V4N

Structure of 5'-deoxy-5'-methylthioadenosine phosphorylase homologue from Sulfolobus tokodaii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-18B
Synchrotron site	Photon Factory
Beamline	BL-18B
Temperature [K]	100
Detector technology	CCD
Collection date	2003-05-27
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0000
Spacegroup name	C 2 2 21
Unit cell lengths	79.869, 142.888, 169.564
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.450
Rwork	0.190
R-free	0.24090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB code 1cg6
RMSD bond length	0.021
RMSD bond angle	2.123
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.540
High resolution limit [Å]	2.450	2.450
Rmerge		0.229
Number of reflections	36084
<I/σ(I)>	13.7
Completeness [%]	100.0	100
Redundancy	6.93	6.81

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	Tris buffer, PEG1000, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K