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1V4N

Structure of 5'-deoxy-5'-methylthioadenosine phosphorylase homologue from Sulfolobus tokodaii

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsPHOTON FACTORY BEAMLINE BL-18B
Synchrotron sitePhoton Factory
BeamlineBL-18B
Temperature [K]100
Detector technologyCCD
Collection date2003-05-27
DetectorADSC QUANTUM 4
Wavelength(s)1.0000
Spacegroup nameC 2 2 21
Unit cell lengths79.869, 142.888, 169.564
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution50.000 - 2.450
Rwork0.190
R-free0.24090
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)PDB code 1cg6
RMSD bond length0.021
RMSD bond angle2.123
Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwareAMoRE
Refinement softwareCNS
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0002.540
High resolution limit [Å]2.4502.450
Rmerge0.229
Number of reflections36084
<I/σ(I)>13.7
Completeness [%]100.0100
Redundancy6.936.81
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP7293Tris buffer, PEG1000, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K

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