1V4E
Crystal Structure of Octaprenyl Pyrophosphate Synthase from Hyperthermophilic Thermotoga maritima
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL12B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL12B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-02-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0093 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 151.530, 151.530, 69.722 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.280 |
| Rwork | 0.226 |
| R-free | 0.28420 * |
| Structure solution method | MIR |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.103 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.360 |
| High resolution limit [Å] | 2.280 | 2.280 |
| Rmerge | 0.059 | 0.423 |
| Total number of observations | 360254 * | |
| Number of reflections | 36239 | |
| <I/σ(I)> | 31.48 | 4.36 |
| Completeness [%] | 96.3 | 95.8 |
| Redundancy | 9.9 | 9.94 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | Na+HEPES, lithium sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Triton X-100 | 0.1 (%) | |
| 3 | 1 | reservoir | sodium HEPES | 0.1 (M) | pH7.5 |
| 4 | 1 | reservoir | 1.5 (M) |
