1V4A

Structure of the N-terminal Domain of Escherichia coli Glutamine Synthetase adenylyltransferase

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2003-02-05
Wavelength(s)	0.95372, 0.97844, 0.97855
Spacegroup name	P 32 2 1
Unit cell lengths	116.836, 116.836, 67.744
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.000
R-factor	0.22993
Rwork	0.228
R-free	0.26900
Structure solution method	MAD
RMSD bond length	0.011
RMSD bond angle	1.076
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.083	0.517
Number of reflections	36424
<I/σ(I)>	8.9	5.2
Completeness [%]	100.0	100
Redundancy	35

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	294	PEG 2000 MME, sodium formate, sodium acetate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K