1V3Y

The crystal structure of peptide deformylase from Thermus thermophilus HB8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2002-04-23
Detector	MAR CCD 165 mm
Wavelength(s)	1.00
Spacegroup name	P 43
Unit cell lengths	62.582, 62.582, 105.271
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.800 - 1.810
R-factor	0.17271
Rwork	0.171
R-free	0.20849
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.018
RMSD bond angle	1.617
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.1.19)

Data quality characteristics

	Overall
Low resolution limit [Å]	19.960
High resolution limit [Å]	1.810
Number of reflections	36563
Completeness [%]	99.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	288	20% PEG 4000, 0.1M Tris-HCl, 200MM Sodium Acetate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 288K