1V3L
Crystal structure of F283L mutant cyclodextrin glycosyltransferase complexed with a pseudo-tetraose derived from acarbose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | MACSCIENCE |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2002-06-24 |
| Detector | BRUKER SMART 6000 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 64.970, 73.710, 80.080 |
| Unit cell angles | 85.47, 105.53, 101.43 |
Refinement procedure
| Resolution | 10.000 - 2.100 |
| R-factor | 0.173 |
| Rwork | 0.169 |
| R-free | 0.23200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pam |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.900 |
| Data reduction software | SAINT |
| Data scaling software | SAINT |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.400 | 1.870 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 83161 | |
| <I/σ(I)> | 1.37 | |
| Completeness [%] | 62.7 | |
| Redundancy | 2.33 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | PEG3000, SODIUM CITRATE, 2-PROPANOL, CALCIUM CHLORIDE, ACARBOSE, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
