1V3H
The roles of Glu186 and Glu380 in the catalytic reaction of soybean beta-amylase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-11-25 |
Detector | MARRESEARCH |
Wavelength(s) | 0.986 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 85.379, 85.379, 143.959 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.810 - 1.600 |
R-factor | 0.163 |
Rwork | 0.163 |
R-free | 0.18100 |
Structure solution method | rigid body refinement |
Starting model (for MR) | 1byb |
RMSD bond length | 0.005 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | CNS (1.1) |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 99.000 | 1.650 |
High resolution limit [Å] | 1.600 | 1.600 |
Number of reflections | 80563 | |
Completeness [%] | 99.3 | 98 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 277 | ammonium sulfate, sodium acetate, 2-mercaptoethanol, EDTA, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 277K |