1V3H
The roles of Glu186 and Glu380 in the catalytic reaction of soybean beta-amylase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-11-25 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.986 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 85.379, 85.379, 143.959 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.810 - 1.600 |
| R-factor | 0.163 |
| Rwork | 0.163 |
| R-free | 0.18100 |
| Structure solution method | rigid body refinement |
| Starting model (for MR) | 1byb |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS (1.1) |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 1.650 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 80563 | |
| Completeness [%] | 99.3 | 98 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 277 | ammonium sulfate, sodium acetate, 2-mercaptoethanol, EDTA, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
