1V3B

Structure of the hemagglutinin-neuraminidase from human parainfluenza virus type III

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-ID-B
Synchrotron site	APS
Beamline	14-ID-B
Temperature [K]	113
Detector technology	CCD
Detector	MARRESEARCH
Spacegroup name	P 62 2 2
Unit cell lengths	219.142, 219.142, 109.379
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.530 - 2.000
R-factor	0.182
Rwork	0.182
R-free	0.21400 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PIV3 HN MONOMER FROM ORTHORHOMBIC STRUCTURE ENTRY 1V2I
RMSD bond length	0.019
RMSD bond angle	26.500 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	190.000 *	2.030
High resolution limit [Å]	2.000 *	2.000
Rmerge	0.170
Total number of observations	1627869 *
Number of reflections	103536
<I/σ(I)>	23	2.9
Completeness [%]	100.0 *	99.9 *
Redundancy	14.7	11.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.5	298	AMMONIUM AND LITHIUM PHOSPHATE, HEPES, pH 7.50, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	Vapor diffusion *	7.5	298	AMMONIUM AND LITHIUM PHOSPHATE, HEPES, pH 7.50, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	HEPES	0.1 (M)	pH7.5
2	1	reservoir	ammonium sulfate	1.0 (M)
3	1	reservoir		1.0 (M)
4	1	drop	protein	2 (mg/ml)