1V35

Crystal Structure of Eoyl-ACP Reductase with NADH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	298
Detector technology	IMAGE PLATE
Detector	RIGAKU
Wavelength(s)	1.5418
Spacegroup name	P 43 21 2
Unit cell lengths	133.315, 133.315, 83.752
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.870 - 2.500
R-factor	0.165
Rwork	0.165
R-free	0.20200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1eno
RMSD bond length	0.028
RMSD bond angle	1.600
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.101	0.390
Number of reflections	26266
<I/σ(I)>	14	5.7
Completeness [%]	98.2	99.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	298	MES, Ammonium Sulphate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K