1V34
Crystal structure of Pyrococcus horikoshii DNA primase-UTP complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU ULTRAX 18 |
Temperature [K] | 90 |
Detector technology | IMAGE PLATE |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 76.386, 76.386, 128.830 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 2.700 |
Rwork | 0.210 |
R-free | 0.26400 * |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.358 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.081 | 0.251 * |
Number of reflections | 12107 | |
<I/σ(I)> | 8.6 | 2.1 |
Completeness [%] | 96.9 | 95.8 |
Redundancy | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | PEG 8000, AMMONIUM SULFATE, POTASSIUM PHOSPHATE, SODIUM CACODYLATE, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 6-10 (mg/ml) | |
2 | 1 | reservoir | PEG8000 | 10.5 (%) | |
3 | 1 | reservoir | ammonium sulfate | 10 (mM) | |
4 | 1 | reservoir | potassium phosphate | 22.5 (mM) | pH7 |
5 | 1 | reservoir | sodium phosphate | 5 (mM) | pH6.5 |