1V33
Crystal structure of DNA primase from Pyrococcus horikoshii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 1999-11-20 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 76.451, 76.451, 128.894 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 * - 1.800 |
| Rwork | 0.219 |
| R-free | 0.24400 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.487 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.048 | 0.176 |
| Number of reflections | 38381 | |
| <I/σ(I)> | 15.2 | 4.2 |
| Completeness [%] | 90.6 | 75.8 |
| Redundancy | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | PEG 8000, AMMONIUM SULFATE, POTASSIUM PHOSPHATE, SODIUM CACODYLATE, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6-10 (mg/ml) | |
| 2 | 1 | reservoir | PEG8000 | 10.5 (%) | |
| 3 | 1 | reservoir | ammonium sulfate | 10 (mM) | |
| 4 | 1 | reservoir | potassium phosphate | 22.5 (mM) | pH7 |
| 5 | 1 | reservoir | sodium phosphate | 5 (mM) | pH6.5 |
