1V30
Crystal Structure Of Uncharacterized Protein PH0828 From Pyrococcus horikoshii
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-04-18 |
Detector | MARRESEARCH |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 51.692, 51.692, 81.511 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 7.000 - 1.400 |
R-factor | 0.217 |
Rwork | 0.217 |
R-free | 0.22900 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 25.300 |
Data reduction software | HKL-2000 |
Data scaling software | SCALA |
Phasing software | SHARP |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 17.300 | 1.480 |
High resolution limit [Å] | 1.400 | 1.400 |
Number of reflections | 25279 | |
<I/σ(I)> | 3.8 | 2.6 |
Completeness [%] | 99.2 | 99.2 |
Redundancy | 10.4 | 10.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 293 | Sodium Citrate , pH 9.50, VAPOR DIFFUSION, HANGING DROP, temperature 293K |