1V30
Crystal Structure Of Uncharacterized Protein PH0828 From Pyrococcus horikoshii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-04-18 |
| Detector | MARRESEARCH |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 51.692, 51.692, 81.511 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 7.000 - 1.400 |
| R-factor | 0.217 |
| Rwork | 0.217 |
| R-free | 0.22900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 25.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.300 | 1.480 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Number of reflections | 25279 | |
| <I/σ(I)> | 3.8 | 2.6 |
| Completeness [%] | 99.2 | 99.2 |
| Redundancy | 10.4 | 10.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 293 | Sodium Citrate , pH 9.50, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
