1V2F

Crystal Structure of T.th HB8 Glutamine Aminotransferase complex with 3-phenylpropionate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-18B
Synchrotron site	Photon Factory
Beamline	BL-18B
Temperature [K]	293
Detector technology	CCD
Collection date	2002-12-19
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0
Spacegroup name	P 31 2 1
Unit cell lengths	123.850, 123.850, 122.500
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 2.350
Rwork	0.188
R-free	0.22200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.350
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.700	2.430
High resolution limit [Å]	2.350	2.350
Number of reflections	45347
Completeness [%]	99.6	99.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	PEG4000, disodium tartrate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K