1V2F
Crystal Structure of T.th HB8 Glutamine Aminotransferase complex with 3-phenylpropionate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-18B |
Synchrotron site | Photon Factory |
Beamline | BL-18B |
Temperature [K] | 293 |
Detector technology | CCD |
Collection date | 2002-12-19 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 123.850, 123.850, 122.500 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 10.000 - 2.350 |
Rwork | 0.188 |
R-free | 0.22200 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.350 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.700 | 2.430 |
High resolution limit [Å] | 2.350 | 2.350 |
Number of reflections | 45347 | |
Completeness [%] | 99.6 | 99.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | PEG4000, disodium tartrate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |