1V2E

Crystal Structure of T.th HB8 Glutamine Aminotransferase complex with a-keto-g-methylthiobutyrate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-18B
Synchrotron site	Photon Factory
Beamline	BL-18B
Temperature [K]	293
Detector technology	CCD
Collection date	2002-12-19
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0
Spacegroup name	P 31 2 1
Unit cell lengths	123.140, 123.140, 122.960
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 2.600
Rwork	0.231
R-free	0.28600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.420
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.700	2.690
High resolution limit [Å]	2.600	2.600
Number of reflections	32897
Completeness [%]	98.5	98.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	PEG4000, disodium tartrate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K